CBSE Class 12 Biotechnology Previous Year Question Paper 2017 (Delhi Set 1)
This is the CBSE Class 12 Biotechnology Previous Year Question Paper from the 2017 Main Exam, Delhi Set 1. The paper is divided into four sections: A, B, C, and D. Questions 1 to 6 are very short answer type, carrying 1 mark each. Questions 7 to 14 are short answer type, carrying 2 marks each. Questions 15 to 25 are also short answer type, carrying 3 marks each, with internal choices provided. Questions 26 to 28 are long answer type, carrying 5 marks each, also with internal choices. All questions are compulsory, and there is no overall choice. Solving this board question paper will help students understand the exam structure, question types, and marking scheme, enabling them to prepare effectively for their board examinations.
Quick info
| Board | CBSE |
|---|---|
| Class | 12 |
| Subject | Biotechnology |
| Session | 2017 |
| Language | English |
| Type | Previous Year Question Paper |
| Exam type | Board Exam |
Paper pattern
The question paper contains four sections (A, B, C, D). It includes very short answer questions (1 mark), short answer questions (2 and 3 marks), and long answer questions (5 marks). Internal choices are provided for 3-mark and 5-mark questions.
Topics covered
Paper topics
- Enzyme inactivation
- Animal cell culture growth phases
- Bacterial vs. viral growth patterns
- Microbial cell culture preservation
- rDNA technology vectors
- Protein concentration measurement
- Expression vectors
- Germplasm conservation limitations
- Eukaryotic protein expression
- Structural genomics
- Functional genomics
- Protein hydrogen bonds
Important topics
- rDNA technology
- Germplasm conservation
- Protein expression
- Genomics
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Question paper text
Question Paper Delhi 2017 set 1 CBSE Class 12 Bio-technology
General Instructions:
All questions are compulsory.
There is no overall choice. However, an internal choice has been provided in questions of three marks and five marks each. You have to attempt only one of the choices in such questions. Question paper contains four sections A, B, C and D.
Questions number 1 to 6 are very short answer questions, carrying 1 mark each.
Questions number 7 to 14 are short answer questions, carrying 2 marks each.
Questions number 15 to 25 are also short answer questions, carrying 3 marks each.
Questions number 26 to 28 are long answer questions, carrying 5 marks each.
Use of calculators is not permitted. However, you may use log tables, if necessary.
SECTION – A
- Natural form of subtilisin enzyme is inactivated by bleach in detergents. How?
Ans. Oxidation of methionine at position 222.
- In which particular phase of growth of animal cell culture, the cell number begins to increase exponentially?
Ans. Log phase/exponential phase.
- Pattern of growth in bacteria is different from viruses. How?
Ans. Bacteria grow by binary fission; viruses do not follow a defined growth pattern.
- Why is strain preservation important in microbial cell culture?
Ans. To ensure availability for future research/viability/to retain metabolite production.
- In rDNA technology, what is the advantage of using vectors with polylinkers or multiple cloning sites?
1/7
Ans. To provide flexibility in use of different restriction enzymes.
- Protein chemists prefer measuring absorbance of samples at 280 nm to estimate protein concentration. Why?
Ans. Nondestructive method.
SECTION - B
- What are expression vectors? What kind of promoter should be used in such vectors?
Ans. Vectors incorporating suitable signals for expressing foreign proteins in the particular host.
- Germplasm conservation through the conventional methods has many limitations.
Name any four.
Ans. Seed dormancy, seed borne diseases, short lived, high cost.
- Generally, eukaryotic hosts are preferred to express eukaryotic proteins. Why?
Ans. Removal of introns/posttranscriptional modifications/ posttranslational modifications/ correct 3-D folding. (any 2)
- Differentiate between structural and functional genomics. (any 2 points)
Ans.
Structural genomics Functional genomics
Involves high throughput DNA sequencing Determination of function of genes
High resolution genetic, physical and transcript Studying interactions between genes map
- How are Hydrogen bonds created in proteins and when are they strongest?
Ans. Sharing of a hydrogen between two electronegative atoms; strongest when the atoms 2/7
Frequently asked questions
What is this document?
This is the CBSE Class 12 Biotechnology Previous Year Board Question Paper from the 2017 Main Exam, Delhi Set 1.
What is the structure of the Biotechnology 2017 paper?
The paper has four sections (A, B, C, D) with questions ranging from 1-mark very short answers to 5-mark long answers, including internal choices for 3 and 5-mark questions.
How can solving this previous year paper help?
Solving this previous year question paper helps students understand the CBSE exam pattern, question difficulty, and marking scheme, leading to better preparation and improved scores.
What topics are covered in this paper?
The paper covers topics such as enzyme inactivation, cell culture, rDNA technology, germplasm conservation, protein expression, and genomics.
Is there any choice available in the questions?
Yes, internal choices are provided in questions carrying 3 marks and 5 marks each, but there is no overall choice in the paper.
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