CBSE Class 12 Biology Board Question Paper 2014
This is the CBSE Class 12 Biology Previous Year Question Paper from 2014, focusing on the Processes of Recombinant DNA Technology. The paper includes questions of varying marks, such as 1-mark questions testing basic knowledge of enzymes used in DNA isolation (lysozyme, chitinase, cellulase) and the definition of transgenic organisms and recombinant proteins. It also features 2-mark questions that delve into the Polymerase Chain Reaction (PCR) technique, asking about the source and properties of DNA polymerase (Taq polymerase from Thermus aquaticus) and the role of primers. Additionally, it touches upon bioreactors, identifying a stirred-tank bioreactor and its purpose in producing gene products. Solving this board question paper helps students familiarize themselves with the exam format, question types, and key concepts in recombinant DNA technology, ultimately aiding in better preparation and performance in their board exams.
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Quick info
| Board | CBSE |
|---|---|
| Class | 12 |
| Subject | Biology |
| Session | 2014 |
| Language | English |
| Type | Previous Year Question Paper |
| Exam type | Board Exam |
Paper pattern
The paper includes 1-mark and 2-mark questions covering topics like DNA isolation, PCR, and bioreactors.
Topics covered
Paper topics
- Recombinant DNA Technology
- Enzymes in DNA Isolation
- Lysozyme
- Chitinase
- Cellulase
- Transgenic Organisms
- Recombinant Proteins
- Polymerase Chain Reaction (PCR)
- DNA Polymerase
- Primers
- Bioreactors
- Stirred-tank bioreactor
- Thermus aquaticus
Important topics
- Enzymes for DNA isolation
- Role of PCR in biotechnology
- Properties of Taq polymerase
- Function of bioreactors
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Question paper text
Processes of Recombinant DNA Technology
1 Mark Questions
- Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
[All India 2014; Foreign 2014]
Ans. The enzymes used for the isolation of DNA from bacterial cells is lysozyme and fungal cells is chitinase.
- How can bacterial DNA be released from the bacterial cell for biotechnology experiments? [Delhi 2011] Ans. Bacterial cells are treated with lysozyme to digest the cell wall for releasing DNA.
- Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells? [Delhi 2010]
Ans. Cellulase is used for digesting the cellulosic cell wall of plant cells. Animal cells do not contain cell wall, so cellulase is not required.
- What is the host called that produce a foreign gene product? What is this product called?
[Foreign 2010]
Ans. The host cells that produce foreign gene product are called transgenic organisms or Genetically Modified Organisms (GMOs). The product is called recombinant proteins.
2 Marks Questions
- Name the source of the DNA polymerase used in PCR technique. Mention why it is used? [All India 2013,2012; Foreign 2011]
Ans. The DNA polymerase used in PCR is Tag polymerase extracted from Thermus aquaticus. It is a thermostable enzyme that can withstand high temperature used in the denaturation and separation of DNA strands. Hence, it can be used for a number of cycles in amplification.
- Name the type of bioreactor shown. Write the purpose for which it is used? [All India 2011]
Ans.Figure is a simple stirred-tank bioreactor.
Bioreactors are used to produce large quantities of the desired gene products
- (i) Mention the number of primers required in each cycle of Polymerase Chain Reaction (PCR). Write the role of primers and DNA polymerase in PCR.
- Give the characteristic feature and source organism of the DNA polymerase used in PCR. [All India 2010]
Ans. (i) Two sets of primers are required. Primers are required for the addition of nucleotides to make multiple copies of the DNA of interest. The enzyme DNA polymerase extends the primers using the nucleotide provided.
- The DNA polymerase is thermostable and withstands the high temperature treatment during denaturation of the DNA
it is obtained from a bacterium, Thermus aquaticus.
Frequently asked questions
What is this document?
This is a CBSE Class 12 Biology Previous Year Question Paper from 2014, designed for board exam practice.
What topics are covered in this paper?
The paper focuses on the Processes of Recombinant DNA Technology, including DNA isolation, Polymerase Chain Reaction (PCR), and bioreactors.
How does solving this previous year paper help?
Solving this previous year question paper helps students understand the exam pattern, question types, and key concepts, improving their preparation and potential scores.
What is the source of DNA polymerase used in PCR?
The DNA polymerase used in PCR is Taq polymerase, sourced from the bacterium Thermus aquaticus.
What are transgenic organisms?
Transgenic organisms, also known as Genetically Modified Organisms (GMOs), are host cells that produce a foreign gene product.
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