CBSE Class 12 Biology Previous Year Question Paper 2014
This document contains the CBSE Class 12 Biology Previous Year Question Paper from 2014, focusing on "Principles of Biotechnology & Tools of Recombination DNA Technology". It includes one-mark questions that test fundamental concepts in biotechnology, such as the requirements for alien DNA replication, suitable host cells for gene guns, components of the first artificial recombinant DNA molecule, and techniques like microinjection and gel electrophoresis. The questions also cover methods for introducing DNA into bacterial cells and the role of cloning vectors. Solving this board question paper provides valuable insights into the exam pattern and helps students reinforce their understanding of key topics, ultimately aiding in better exam preparation and performance.
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Quick info
| Board | CBSE |
|---|---|
| Class | 12 |
| Subject | Biology |
| Session | 2014 |
| Language | English |
| Type | Previous Year Question Paper |
| Exam type | Board Exam |
Paper pattern
The paper includes one-mark questions testing specific concepts in Biotechnology and Recombinant DNA Technology.
Topics covered
Paper topics
- Recombinant DNA Technology
- Biotechnology
- Cloning Vectors
- Gene Introduction Techniques
- Gel Electrophoresis
- Agrobacterium tumefaciens
Important topics
- Origin of Replication
- Gene Guns
- Microinjection
- Gel Electrophoresis Matrix
- Cloning Vector Uses
- Agrobacterium as Natural Genetic Engineer
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Question paper text
Principles of Biotechnology & Tools of Recombination DNA Technology
1 Mark Questions
- Why is it not possible for an alien DNA to become part of a chromosome anywhere along its length and replicate normally?
[All India 2014]
Ans. The integration of alien DNA is required to become a part of chromosome. As the DNA itself cannot multiply and replicate but rather requires a specific sequence for initiating its replication called origin of replication. Therefore, the alien DNA needs to be joined with the host DNA with the help of enzymes and linked to Ori, so as to be part of chromosome and replicate normally to produce its copies.
2. Mention the type of host cells suitable for the gene guns to introduce an alien DNA. [Delhi 2014]
Ans. The host cells suitable for the gene guns to introduce an alien DNA are plant cells.
- Write the two components of first artificial recombinant DNA molecule constructed by Cohen and Boyer. [Foreign 2014]
Ans. The two components of first artificial recombinant DNA molecule constructed by Gohen and Boyer are:
- antibiotic resistance gene
- plasmid of Salmonella typhimurium
4. Name the host cells in which microinjection technique is used to introduce an alien DNA. [Foreign 2014]
Ans. The microinjection technique to introduce alien DNA is usually carried out in animal cell, i.e. directly into the nucleus.
- Name the material used as matrix in gel electrophoresis and mention its role. [All India 2014C]
Ans. The material used as matrix in gel electrophoresis is agarose. This agarose gel acts as a sieve to separate the DNA fragments according to their size.
- Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments. [All India 2013]
Ans. Ways to introduce desired DNA into bacterial cell are:
- microinjection (ii) disarmed pathogen vectors
- portion by bivalent cation such as calcium
- bidlistic or gene gun
- State what happens when an alien gene is ligated at Sal I site of pBR322 plasmid. [Delhi 2013c]
Ans. If an alien gene is ligated at Sal I site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid will lose its tetracycline resistance.
- Mention the uses of cloning vector in biotechnology? [Delhi 2011] Ans. Uses of cloning vector in biotechnology.
- Helps in linking the foreign/alien DNA with that of host's DNA.
- Help in the selection erf recombinants from the non-recombinants.
- Biotechnologists refer to Agrobacterium tumefaciens as natural genetic engineer of plants. Give reasons to support the statement. [HOTS; All India 2011] Ans. Agrobacterium tumefacieps is a pathogen of several dicot plants. It is used as a
Frequently asked questions
What is this document?
This is a CBSE Class 12 Biology Previous Year Question Paper from 2014, focusing on Principles of Biotechnology and Tools of Recombination DNA Technology.
What is the benefit of solving this paper?
Solving this previous year question paper helps students understand the exam pattern, practice answering questions on key biotechnology concepts, and improve their marks in the CBSE board exams.
What topics are covered in this paper?
The paper covers topics like the replication of alien DNA, gene introduction techniques (gene gun, microinjection), components of recombinant DNA, gel electrophoresis, cloning vectors, and the role of Agrobacterium tumefaciens.
What is the format of the questions?
This section of the paper features one-mark questions that require concise answers, testing specific knowledge points in biotechnology.
How can this paper help in exam preparation?
By practicing with this 2014 board question paper, students can assess their knowledge, identify weak areas, and get familiar with the types of questions asked by the CBSE for Class 12 Biology.
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